Draft:DNA programmed assembly of cells

== Related research ==

== Related research ==

DNA-programmed cell assembly builds on and is interconnected with several broader areas of research, including DNA [[nanotechnology]], programmable materials, and synthetic biology. DNA origami is a more specific example of a programmable structural material, in which a long ssDNA scaffold can be contorted into complex 2D and 3D shapes through short complementary staple strands. Several orthogonal DNA pairs interact with each other to create complex structures, usually in the [[nanometer]] range.{{Cite journal |last1=Hong |first1=Fan |last2=Zhang |first2=Fei |last3=Liu |first3=Yan |last4=Yan |first4=Hao |date=2017-10-25 |title=DNA Origami: Scaffolds for Creating Higher Order Structures |url=https://pubs.acs.org/doi/10.1021/acs.chemrev.6b00825 |journal=Chemical Reviews |language=en |volume=117 |issue=20 |pages=12584–12640 |doi=10.1021/acs.chemrev.6b00825 |pmid=28605177 |issn=0009-2665}} DNA-origami nanoarrays can be used with multivalent aptamers to improve binding affinity to low-affinity [[antigen]]-specific cells through adjusting aptamer valency and spacing to match the target proteins' surfaces. Additionally, short oligonucleotide strands called aptamers can bind to proteins with high affinity and specificity, making them useful for targeted protein degradation.{{Cite journal |last1=Liu |first1=Yuan |last2=Qian |first2=Xu |last3=Ran |first3=Chunyan |last4=Li |first4=Longjie |last5=Fu |first5=Ting |last6=Su |first6=Dan |last7=Xie |first7=Sitao |last8=Tan |first8=Weihong |date=2023-04-11 |title=Aptamer-Based Targeted Protein Degradation |url=https://pubs.acs.org/doi/10.1021/acsnano.2c10379 |journal=ACS Nano |language=en |volume=17 |issue=7 |pages=6150–6164 |doi=10.1021/acsnano.2c10379 |pmid=36942868 |bibcode=2023ACSNa..17.6150L |issn=1936-0851}} DPAC has also been used in the design of microfluidic devices. Chambers with complementary ssDNA strands can immobilize modified cells, allowing for analysis of non-adherent cells with single-cell precision.{{Cite journal |last1=Douglas |first1=Erik S. |last2=Hsiao |first2=Sonny C. |last3=Onoe |first3=Hiroaki |last4=Bertozzi |first4=Carolyn R. |last5=Francis |first5=Matthew B. |last6=Mathies |first6=Richard A. |date=2009 |title=DNA-barcode directed capture and electrochemical metabolic analysis of single mammalian cells on a microelectrode array |journal=Lab on a Chip |language=en |volume=9 |issue=14 |pages=2010–2015 |doi=10.1039/b821690h |issn=1473-0197 |pmc=2892333 |pmid=19568668}}

DNA-programmed cell assembly builds on and is interconnected with several broader areas of research, including DNA [[nanotechnology]], programmable materials, and synthetic biology. DNA origami is a more specific example of a programmable structural material, in which a long ssDNA scaffold can be contorted into complex 2D and 3D shapes through short complementary staple strands. Several orthogonal DNA pairs interact with each other to create complex structures, usually in the [[nanometer]] range.{{Cite journal |last1=Hong |first1=Fan |last2=Zhang |first2=Fei |last3=Liu |first3=Yan |last4=Yan |first4=Hao |date=2017-10-25 |title=DNA Origami: Scaffolds for Creating Higher Order Structures |url=https://pubs.acs.org/doi/10.1021/acs.chemrev.6b00825 |journal=Chemical Reviews |language=en |volume=117 |issue=20 |pages=12584–12640 |doi=10.1021/acs.chemrev.6b00825 |pmid=28605177 |issn=0009-2665}} DNA-origami nanoarrays can be used with multivalent aptamers to improve binding affinity to low-affinity [[antigen]]-specific cells through adjusting aptamer valency and spacing to match the target proteins' surfaces. Additionally, short [[oligonucleotide]] strands called aptamers can bind to proteins with high affinity and specificity, making them useful for targeted protein degradation.{{Cite journal |last1=Liu |first1=Yuan |last2=Qian |first2=Xu |last3=Ran |first3=Chunyan |last4=Li |first4=Longjie |last5=Fu |first5=Ting |last6=Su |first6=Dan |last7=Xie |first7=Sitao |last8=Tan |first8=Weihong |date=2023-04-11 |title=Aptamer-Based Targeted Protein Degradation |url=https://pubs.acs.org/doi/10.1021/acsnano.2c10379 |journal=ACS Nano |language=en |volume=17 |issue=7 |pages=6150–6164 |doi=10.1021/acsnano.2c10379 |pmid=36942868 |bibcode=2023ACSNa..17.6150L |issn=1936-0851}} DPAC has also been used in the design of microfluidic devices. Chambers with complementary ssDNA strands can immobilize modified cells, allowing for analysis of non-adherent cells with single-cell precision.{{Cite journal |last1=Douglas |first1=Erik S. |last2=Hsiao |first2=Sonny C. |last3=Onoe |first3=Hiroaki |last4=Bertozzi |first4=Carolyn R. |last5=Francis |first5=Matthew B. |last6=Mathies |first6=Richard A. |date=2009 |title=DNA-barcode directed capture and electrochemical metabolic analysis of single mammalian cells on a microelectrode array |journal=Lab on a Chip |language=en |volume=9 |issue=14 |pages=2010–2015 |doi=10.1039/b821690h |issn=1473-0197 |pmc=2892333 |pmid=19568668}}

==References==

==References==